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Ethanol extracts of Boerhavia diffusa and Costus afer and their combination were tested for their effects on haematological parameters of broiler chicken and possible synergistic effect due to their combination. The parameters were Red Blood Cell count, White Blood Cell count, Platelet count, Blood Glucose Packed Cell Volume, Haemoglobin content and Electrolyte concentration. At the end of the analysis period of four weeks, it was found that B. diffusa extract and the combination of the two extracts increased red blood cell count significantly above the control and those fed with C. afer extract. However, C. afer extract increased the white blood cell count from 10.1 to 13.1 x 1012, B. diffusa extract from 10.7 to 11.8 x 1012 and the combination from 11.5 to 12 x 1012. The control value remained constant. All the extracts, including the combination increased platelet count significantly above the control. Blood glucose was most significantly reduced by the combination of the two extracts, while other samples including the control showed constant values. The packed cell volume (PCV) and haemoglobin content showed a drop in value in all samples except those fed a combination of the two extracts, which increased PCV value from 32-33% and increased haemoglobin values from 10.7 to 11g/dl. Electrolyte concentration at the end of research period ranged from 97.8 to 104.5 for chloride, 3.6 to 4.3 for potassium ion and 138.4 to 142.6 for sodium ion.
Alikwe, P. C., Faremi, A. V., and Egwaikhide, P. A. (2010). Biochemical evaluation of serum metabolites and haematological indices of broiler chicks fed with varying levels of rumen epithelial scraps in place of fish meal protein. EJEAFChe 9(6):991-999
Al-Kassae, G. A. M. (2009). Influence of two plant extracts derived from thyme and cinnamon on broiler performance. Pakistan Veterinary Journal 29(4):169-173
A. O. A. C. (1990). Official Methods of Analyses of the Association of Analytical Chemists, Washington D.C.
AOAC. (1984). Official Methods of Analysis of the Association of Analytical Chemists, Washington D.C.
Anaga, A. O., Njoku, C. J., Ekejiuba, E. S., Esiaka, M. N., and Asuzu, I. U. (2004). Investigations of the methanolic leaf extract of Costus afer Ker-Gawl for pharmacological activities in vitro and in vivo. http://www.thefreelibrary.com
Ansari, J. Z., Haq, A., Yousaf, M., Ahmad, T., and Khan, S. (2008). Evaluation of different medicinal plants as growth promoters for broiler chicks. Sarhad Journal of Agriculture 24 (2): 323-333
Anyasor, G. N., Ogunwenmo, K. O., Oyelana, O. A., and Akpofunure, B. E. (2010). Phytochemical constituents and anti-oxidant activities of aqueous and methanol stem extracts of Costus afer Ker Gawl. African Journal of Biotechnology 9(31):4880-4884
Awasth, L. P., and Verma, H. N. (2006). Boerhavia diffusa A wild herb with potent biological and antimicrobial properties. Asian Agri-History 10: 55–68 http://www.agri-history.org/pdf/boerhavia.pdf
Barreto, M. S., Mentea, J. F., Racaniccu, A. M., Perriera, P. M., and Rizzo, P. V. (2008). Plant extracts used as growth promoters in broiler chickens. Rev. Bras. Cienc. Avic. 10(2):109- 115
Biu, A. A., Yusufu, S. D., and Rabo, J. S. (2009). Studies on the effect of aqueous leaf extracts of neem (Azadirachta indica A. Juss) on haematological parameters in chicken. African Scientist 10(4):189-192
Durrani, F. R., Abdulahi, N., Chand, N., Durrani, Z., and Akhtar, S. (2008). Haematological biochemical immunomodulatory and growth promoting effect of feed added wild mint (Mentha longifolia) in broiler chicks. Sarhad Journal of Agriculture 24(4):661 – 664
Eisa, A. M., and Sohar, A. H. (2003). Clinicopathological Studies on biostimulant agent in Broiler Chickens. Rafr El-Sheikl Veterinary Medicine Journal 1(1):631-644
Fajimi, A. K., and Taiwo, A. A. (2005). Herbal remedies in animal parasitic diseases in Nigeria: a review. African Journal of Biochemistry 4(4): 303 – 307
Harbone, J. B. (1998). Methods of extraction and isolation. Phytochemical methods Chapman and Hall, London.
Harbone, J. B. (1973). Phytochemical Methods. Chapman and Hall, London p 113
Iwu, M. M. (1983). Traditional Igbo medicine, Institute of African Studies Publication, University of Nigeria Nsukka pp 112 – 144.)
James, C. S. (1995). Analytical Chemistry of Foods. Chapman and Hall, New York
Joy, P. P., Thomas, J., Matthew, S., and Skaria, B. P. (1998). Medicinal Plants. Kerala Agricultural University Publication pp 1-21
Julian, R. J. (2005). Production and growth related disorders and other metabolic diseases of poultry – A review. The Veterinary Journal 169 (2005):350-369
Nwokocha, N. J., Obasi, K. O., Avoaja, A. G. I., and Nwokocha, J. V. (2010). Proximate analysis of some selected Nigerian vegetables. Journal of Natural Science 2(1):71-77
Nworgu, J. C., Ogungbeniro, S. A., and Solesi, K. S. (2007). Performance and some blood chemistry indices of broiler chickens served fluted pumpkin (T. occidentalis) leaf extract supplement. America-Eunsor Journal of Environmental Sciences 2(1):90 – 98.
Ocak, N., Erener, G., Burak, A. K., Sungu, M., Altop, A., and Ozern, A. (2008). Performance of broilers fed diet supplemented with dry peppermint (Mentha piperita) or thyme (Thymus vulgaris) leaves as growth promoter source. Czech Journal of Animal Science 53(4):169-175
Okoli, I. C., Ndujihe, G. E., Ogbuewu, I. P. (2006). Frequency of Isolation of Salmonella from commercial poultry feeds and their antimicrobial resistance profiles in Imo State, Nigeria. Online Journal of Health and Allied Sciences. 5: 1-10
Pagari, P., Abimiku, J. E., Emeka-Okolo, W. (2008). Assessment of the Nigerian Poultry market chain to improve biosecurity. Food and Agricultural Organization of the United Nations Report pp 1-65
Pearson, D. (1996). Chemical Analysis of Foods. Churchill Livingstone, Edingburg United Kingdom. pp 714
Polat, U., Yesilbag, D., and Eren, M. (2011). Serum biochemical profile of broiler chickens fed diets containing rosemary and rosemary volatile oil. Journal of Biological and Environmental Science 5(3):23-30
Saber, K. A. (2011). Influence of two herbal extracts on performance, carcass quality and blood parameters in broiler chickens. Annals of Biological Research 2(5):584-588
Vikari, A. (2009). Insights into the efficiency and effectiveness of plant extracts in broiler and layer nutrition. FBA Issue 29
Windisch, W., Schedle, K., Plitzner, C., and Kroismayr, A. (2007) Use of Phytogenic products as feed additives for swine and poultry. Journal of Animal Science 86:140 – 148.
Yakubu, A. (2010). Indigenous Chicken Flocks of Nassarawa State, Nigeria: Their characteristics, Husbandry and Productivity. Tropical and Subtropical Agroecosystems 12 (1):69-76.
In-Text Citation: (Nwokocha et al., 2013)
To Cite this Article: Nwokocha, J. V., Nwokocha, N. J., Ogbuji, O., Ukpabi, C. I., & Egere, M. S. (2013). Studies on the Effect of the Combination of Boerhavia Diffusa and Costus Afer Leaf extracts on the Haematological Parameters of Broiler Chickens. International Journal of Academic Research in Progressive Education and Development, 2(3), 135–145.
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